Journal of Medicinal and Chemical Sciences

Original Article

The Effectiveness of Epigallocatechin Gallate (EGCG) Eye Drops to Interleukin -1ß and Matrix Metalloproteinase (MMP)-9 Expression in Pseudomonas Aeruginosa Keratitis: An In Vitro Approach

Tarosa Yodia Urolita; Nurwasis .; Ismi Zuhria; Fariztah Sukainah Nur Fathimah; Annisa Karima; Nila Kurniasari; Djoko Agus Purwanto; Eko Budi Koendhori

Volume 7, Issue 3 , March 2024, Pages 461-473

https://doi.org/10.26655/JMCHEMSCI.2024.3.1

Abstract

Introduction: Pseudomonas aeruginosa keratitis is the most prevalent eye infection, known to potentially lead to endophthalmitis and corneal perforation. Although antibiotic therapy ... Read More Introduction: Pseudomonas aeruginosa keratitis is the most prevalent eye infection, known to potentially lead to endophthalmitis and corneal perforation. Although antibiotic therapy effectively eliminates the bacteria, the damage induced by toxins in the cornea persists, resulting in corneal thinning, perforation, or fibrosis. The healing process of the corneal wound is influenced by Interleukin-1β and matrix metalloproteinase-9 (MMP-9). Epigallocatechin gallate (EGCG), possessing antibacterial, anti-inflammatory, and antioxidant properties found in Camellia sinensis extract, holds promise as an adjuvant therapy for bacterial keratitis. This study aims to analyze the expression of Interleukin-1β and matrix metalloproteinase-9 (MMP-9) in a keratitis model induced by Pseudomonas aeruginosa infection following the administration of Epigallocatechin Gallate (EGCG) eye drops (an experimental study conducted on Rattus norvegicus rat models).Methods: The experimental study utilized samples of Rattus norvegicus corneas, previously induced to form keratitis models, with 6 samples each in the negative control group, Moxifloxacin group, and combined Moxifloxacin and EGCG group. Results: The percentage of Interleukin-1β expression in the combined EGCG and Moxifloxacin group was lower compared to the Moxifloxacin group. The results indicated a significant difference in the Interleukin-1β expression among the test groups (p < 0.05). The post hoc test revealed significant differences between the Moxifloxacin group and the combined EGCG group (p < 0.05), as well as between the negative control group and the combined EGCG group (p < 0.05). The mean percentage of MMP-9 expression in the combined EGCG group was lower than that in the Moxifloxacin group. The One-way ANOVA test indicated a significant difference among the three treatment groups (p < 0.05).Conclusion: There was a significant reduction in the expression of Interleukin-1β and MMP-9 in animal models of keratitis induced by Pseudomonas aeruginosa infection following the administration of Epigallocatechin Gallate (EGCG) eye drops.

Case Report

Gynecomastia Caused by Antiretroviral Therapy, Highly Suspected Efavirenz: A Case Report

Prasida Mustika Indarwati; Jongky Hendro Prajitno

Volume 7, Issue 3 , March 2024, Pages 474-481

https://doi.org/10.26655/JMCHEMSCI.2024.3.2

Abstract

Gynecomastia is an enlargement of breasts in men. This disease is commonly found in patients with impaired liver function and patients on additional hormonal use. Approximately, 10% ... Read More

Original Article

Effect of Platelet-Rich Fibrin (PRF) Membrane on the Expression of Matrix Metalloproteinase 9 (MMP-9) and Tissue Inhibitor of Metalloproteinase 1 (TIMP-1) in Corneal Wound Healing: Pseudomonas aeruginosa Keratitis

Oggy Satriya Putra; Luki Indriaswati; Yulia Primitasari; Nandang Sudrajat; Bimanda Rizki Nurhidayat; Mahmudah Mahmudah; Thomas Valentinus Widiyatno

Volume 7, Issue 3 , March 2024, Pages 482-491

https://doi.org/10.26655/JMCHEMSCI.2024.3.3

Abstract

Background: Pseudomonas aeruginosa is the most common cause of bacterial keratitis, with potential complications such as corneal perforation and endophthalmitis. Platelet-rich fibrin ... Read More Background: Pseudomonas aeruginosa is the most common cause of bacterial keratitis, with potential complications such as corneal perforation and endophthalmitis. Platelet-rich fibrin (PRF) membrane is utilized as a treatment for corneal ulcers. Maintaining a balance between collagen formation and extracellular matrix remodeling is crucial in fibrosis, a process regulated by matrix metalloproteinases (MMPs) and Tissue Inhibitors of Matrix Metalloproteinases (TIMPs). This study aims to analyze the effect of the PRF membrane on the expression of MMP-9 and TIMP-1 in keratitis.Methods: This study employed an experimental design with a randomized posttest-only control group. New Zealand white rabbits (Oryctolagus cuniculus) were used as animal models with Pseudomonas aeruginosa keratitis. Levofloxacin eye drops were administered to both the control and treatment groups. However, in the treatment group, a PRF membrane was sutured. The expression of MMP-9 and TIMP-1 was compared between the two groups.Results: The animal models consisted of 12 New Zealand white rabbits, divided into a control group and a treatment group, each comprising 6 rabbits. The mean ± SD of MMP-9 in the treatment group was 2.58 ± 0.72, whereas in the control group, it was 4.62 ± 1.56. TIMP-1 data in the treatment group had a median of 4.10 (with a minimum of 2.00 and a maximum of 4.80), while in the control group, it was 4.80 (with a minimum of 4.20 and a maximum of 8.96). Analysis using the independent t-test and Mann-Whitney test revealed substantial disparities in the expression levels of MMP-9 and TIMP-1 between the control and treatment groups, with p-values of 0.016 and 0.036, respectively. The treatment group exhibited a lower expression of MMP-9 compared to the control group, whereas the treatment group demonstrated a higher expression of TIMP-1 in comparison to the control group.Conclusion: PRF membranes can modulate MMP-9 expression and the balance between MMP-9 and TIMP-1 in degrading and depositing the extracellular matrix (ECM). They can be considered as an option for cases of Pseudomonas aeruginosa keratitis to reduce corneal scarring.

Case Report

Epidural Anesthesia in Pregnant Women with a Total AV Block and Preeclampsia: A Case Report

Herry Cahya Fathani; Fajar Perdhana; Teuku Aswin Husain

Volume 7, Issue 3 , March 2024, Pages 492-499

https://doi.org/10.26655/JMCHEMSCI.2024.3.4

Abstract

Introduction: Total atrioventricular (AV) block presents significant challenges in managing pregnancy and childbirth due to physiological changes that can lead to cardiovascular decompensation, ... Read More

Original Article

Comparison of the Diagnostic Performance of Platelet Aggregation Test using Light Transmission Aggregation (LTA) Method

Chiquita Hasri Mirandari; Yulia Nadar Indrasari; Muhamad Robiul Fuadi; Mohammad Saiful Ardhi

Volume 7, Issue 3 , March 2024, Pages 500-507

https://doi.org/10.26655/JMCHEMSCI.2024.3.5

Abstract

Background: Antithrombotic therapy, such as acetylsalicylic acid (ASA) and clopidogrel, may interfere with platelet aggregation in thrombotic strokes. Therefore, a platelet aggregation ... Read More

Original Article

The Analysis of the Quality of Life in Indonesia in the Middle of the COVID-19 Pandemic: A Cross-Sectional Study

N Juni Triastuti; Erna Herawati

Volume 7, Issue 3 , March 2024, Pages 508-517

https://doi.org/10.26655/JMCHEMSCI.2024.3.6

Abstract

Background: It is widely known that COVID-19 has influenced many aspects of human life. Notwithstanding, the quality of life of the human population in South East Asia, especially in ... Read More

Original Article

Bruguirea gymnorrhyza Leaf Extract Metabolites: Oral Bioavailability and GI Absorption Predictions

Nur Kuswanti; Nur Qomariyah; Erlix Rakhmad Purnama; Firas Khaleyla

Volume 7, Issue 3 , March 2024, Pages 518-529

https://doi.org/10.26655/JMCHEMSCI.2024.3.7

Abstract

Background: Bruguirea gymnorrhyza is used to treat some diseases traditionally. Some scientific studies are important for its explanation and drug development. The aims of this ... Read More

Original Article

Association Between Eating Disorders and the Survival in Older Patients with Dementia: Paliative Care Unit-Based Cohort Study

Tamar Lazashvili; Vera Kapetivadze; Teimuraz Silagadze; Revaz Tabukashvili; Zviadi Maglapheridze; Irina Kapetivadze

Volume 7, Issue 3 , March 2024, Pages 530-537

https://doi.org/10.26655/JMCHEMSCI.2024.3.8

Abstract

The aim of our study was to identify the peculiarities of eating disorders on the survival rates of patients with dementia. The present, prospective cohort study conducted in palliative ... Read More

Original Article

Investigation of Calcium Carbonate Porogen on Methylene Blue Adsorption in Alginate Cellulose Xanthate Beads from Corn Stalks

Eny Yulianti; Nanang Qazim; Anton Prasetyo; Siska Ela Kartika; Mujahidin Ahmad; Rahadian Zainul; Azril Azril; Herland Satriawan; Ahmad Zikri; Mohammad Abdullah

Volume 7, Issue 3 , March 2024, Pages 538-554

https://doi.org/10.26655/JMCHEMSCI.2024.3.9

Abstract

Corn stalk can be used as a potential adsorbent because of its abundance, cost-effectiveness, and accessible functional groups that allow chemical modifications. This study aims to ... Read More

Original Article

Correlation Between Hospital Stakeholder Collaboration and Outpatient Department Performance: A Case Study of One Indonesian Hospital

Purwaningsih Purwaningsih; Nyoman Anita Damayanti; Nasronudin Nasronudin; Mahmudah Mahmudah; Sri Andarini; Mochammad Bagus Qomaruddin; Djazuly Chalidyanto; Slamet Riyadi Yuwono; Aby Nugrah Septanto; Hakim Zulkarnain

Volume 7, Issue 3 , March 2024, Pages 555-564

https://doi.org/10.26655/JMCHEMSCI.2024.3.10

Abstract

Introduction: The emerging concept of stakeholder collaboration in healthcare settings enhance evidence-based practice, patient centered, decision-making, and ultimately, patient outcomes. ... Read More

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Volume & Issue: Volume 7, Issue 3, March 2024, Pages 461-564

The Effectiveness of Epigallocatechin Gallate (EGCG) Eye Drops to Interleukin -1ß and Matrix Metalloproteinase (MMP)-9 Expression in Pseudomonas Aeruginosa Keratitis: An In Vitro Approach

Abstract

Gynecomastia Caused by Antiretroviral Therapy, Highly Suspected Efavirenz: A Case Report

Abstract

Effect of Platelet-Rich Fibrin (PRF) Membrane on the Expression of Matrix Metalloproteinase 9 (MMP-9) and Tissue Inhibitor of Metalloproteinase 1 (TIMP-1) in Corneal Wound Healing: Pseudomonas aeruginosa Keratitis

Abstract

Epidural Anesthesia in Pregnant Women with a Total AV Block and Preeclampsia: A Case Report

Abstract

Comparison of the Diagnostic Performance of Platelet Aggregation Test using Light Transmission Aggregation (LTA) Method

Abstract

The Analysis of the Quality of Life in Indonesia in the Middle of the COVID-19 Pandemic: A Cross-Sectional Study

Abstract

Bruguirea gymnorrhyza Leaf Extract Metabolites: Oral Bioavailability and GI Absorption Predictions

Abstract

Association Between Eating Disorders and the Survival in Older Patients with Dementia: Paliative Care Unit-Based Cohort Study

Abstract

Investigation of Calcium Carbonate Porogen on Methylene Blue Adsorption in Alginate Cellulose Xanthate Beads from Corn Stalks

Abstract

Correlation Between Hospital Stakeholder Collaboration and Outpatient Department Performance: A Case Study of One Indonesian Hospital

Abstract