Journal of Medicinal and Chemical Sciences

Study of Antimicrobial Activity of Silver Nanoparticles Against Salmonella Typhi Infections in Vitro

Rawaa Asad Salman; Sanaa Khudhur Jameel; Sinaa Mahdi Shakir

Volume 6, Issue 4 , April 2023, , Pages 733-745

https://doi.org/10.26655/JMCHEMSCI.2023.4.4

Abstract

The aim of this study is to investigate the antibacterial effect of silver nanoparticles (AgNPs) against multidrug-resistant Salmonella typhi recovered from blood, stool, and fluid ... Read More The aim of this study is to investigate the antibacterial effect of silver nanoparticles (AgNPs) against multidrug-resistant Salmonella typhi recovered from blood, stool, and fluid specimens. In vitro the antimicrobial activity of silver nanoparticles against S.typhi isolates done by determination of silver nanoparticles activity by agar wells diffusion assay, MIC, sub MIC, and by Microtiter plate (Submic). By agar wells diffusion assay and MIC, the antimicrobial activity of silver nanoparticles showed an inhibition zone, among 50 strains of S.typhi. Five Submic showed that sensitivity was higher among Submic 1, 2, and 3 than those in Submic 4 and 5 that show higher resistance (96%, 94%, and 86% vs. 4%), respectively. By Microtiter plate when we used three diluted concentrations of silver nanoparticles of Submic 1, 2, and 3, significant were found that after 24 hours was significantly higher than that after 48 hours, as Submic 1 (0.19874 ± 0.034156 vs. 0.14864 ±0.025908), respectively with mean differences of 0.050100 (t= 9.794, df: 49, P=0.000) and the means of Submic 2 and 3 was (0.20776 ± 0.031197 vs. 0.15730 ± 0.027060) (t=10.401, df: 49, P= 0.000) and (0.21464 ± 0.030793 vs. 0.16802 ± 0.026111) with mean differences of 0.050460 and 0.046620, respectively and (t= 9.031, df: 49, P= 0.000, respectively. Antibacterial activity of silver nanoparticles has been demonstrated in several investigations, but the reported MIC values range through a wide extent of variation. In our study, silver nanoparticles showed a good antibacterial activity against all the tested pathogens. The results of MIC and Submic tests revealed a higher MIC value for S.typhi compared with the other tested pathogens. It is possible that AgNPs act similarly to the antimicrobial agents used for the treatment of MDR S.typhi infections, which show four different mechanisms of action including: interference with cell wall synthesis, inhibition of protein synthesis, interference with nucleic acid synthesis, and inhibition of the metabolic pathway.

Investigation of Biofilm Formation and Antibiotic Resistant of Bacteria Isolated from Septic Neonates

Aliaa Monther Ebrahem; Sanaa Khudhur Jameel; Ali Hafedh Abbas

Volume 6, Issue 4 , April 2023, , Pages 816-826

https://doi.org/10.26655/JMCHEMSCI.2023.4.13

Abstract

Neonatal sepsis refers to the bacterial bloodstream infections of the newborn during the neonatal period as usually the first twenty-eight days of life. The current study was done in ... Read More

Evaluation of Urinary Tract Infection and Measurement of Interleukin 18 in Iraqi Patients with Rheumatoid Arthritis

Esraa Mohammed Jasim; Sanaa Khudhur Jameel; Nabaa Ihsan Awadh

Volume 6, Issue 2 , February 2023, , Pages 440-448

https://doi.org/10.26655/JMCHEMSCI.2023.2.24

Abstract

Although the etiology of RA is complicated, infection with various microorganisms can activate several immune system pathways, potentially leading to an autoimmune response. Urinary ... Read More

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Author = Sanaa Khudhur Jameel

Study of Antimicrobial Activity of Silver Nanoparticles Against Salmonella Typhi Infections in Vitro

Abstract

Investigation of Biofilm Formation and Antibiotic Resistant of Bacteria Isolated from Septic Neonates

Abstract

Evaluation of Urinary Tract Infection and Measurement of Interleukin 18 in Iraqi Patients with Rheumatoid Arthritis

Abstract