Effect of Temperature of Water Used For Reconstitution On Stability Of Antibiotic Dry Suspension

Document Type: Original Article


1 Tripoli Medical Centre, Tripoli-Libya

2 Faculty of Pharmacy, University of Tripoli, Tripoli-Libya


This study is carried out to test the stability of antibiotic dry suspensions reconstituted with various water temperatures. The testing included physical, microbial and chemical changes of samples reconstituted with water at 40, 60, 70 and 80ᵒC. These changes were compared to control samples prepared with water at temperature 25ᵒC. HPLC method was followed qualitatively to identify antibiotic active constituent in addition to quantitative analysis to evaluate antibiotic contents compared to control samples. The changes were assessed within one hour after reconstitution and after four days of reconstitution. Physical tests showed changes of amoxicillin\clavulenic acid suspension’s colour prepared at 80ᵒC. Sedimentation ratio, sedimentation rate and sedimentation volume decreased as temperature increased were these parameters were tested only for amoxicillin suspension. Upon centrifugation there was a decrease in sediment volume accompanied with an increase in supernatant volume resulting in changes in sediment/supernatant ratio. Microbial study showed a marked decrease in antimicrobial activity for both amoxicillin and amoxicillin/clavulenic acid suspensions. HPLC results showed a decrease in amoxicillin and clavulanate in samples prepared with heated water compared to those prepared with cooled water at 25ᵒC.

Graphical Abstract

Effect of Temperature of Water Used For Reconstitution On Stability Of Antibiotic Dry Suspension


Main Subjects

1. P. Nwokoye, O. Oyetunde, M. Akinleye. J. App. Pharm. Sci., 2012, 2:28

2. C.N. Stanley, S.E. Igala. J. Pharm. Res. Int., 2017, 20:1

3. K.K. Naidoo. S.A. Fam, Pract., 2006, 6:48

4. ME Aulton. Pharmaceutics, The science of dosage form design. Churchill Livingstone, 1988, the first edition. The British Pharmacopeia (1995). The Unites State Pharmacopeia (2000).

5. M. Asif, J. Chem. Rev., 2019, 1:47.

6.  S. Sajjadifar, H. Hamidi, K. Pal, J. Chem. Rev., 2019, 1:35.

7.  E.R. Nezhad, S. Sajjadifar, Z. Abbasi, S. Rezayati, Journal of Sciences, Islamic Republic of Iran, 2014, 25:127.

8. D.F. Latona, Adv. J. Chem. A, 2019, 2:225.

9. W.E. John, A.A. Ayi, C. Anyama, P.B. Ashishie, B.E. Inah, Adv. J. Chem. A, 2019, 2:175.

10. M. Kazemi, S. Sajjadifar, A. Aydi, M.M. Heydari, J. Med. Chem. Sci., 2018, 1:1.

11. S.A.A. Shawai, , J. Med. Chem. Sci., 2019, 2:96.