Microbial infection and microbial resistance to the classical antibiotics have been immense challenges which portend the health of societies. This has directed researchers to straight their attention onto the plants to discover new possible antimicrobial compounds. In this work, 5 solvents were employed to extract the dried seeds powder of quince by ultrasound including ethanol, ethyl-acetate, chloroform, n-hexane, and diethyl-ether. Each extract was subjected to three in vitro antimicrobial tests using the broth-dilution method. The antibacterial effect against the following aerobic bacteria which are Pseudomonas aeruginosa ATCC 27853, Klebsiella pneumonia ATCC 700603, Haemophilus influenzae ATCC 49247, Escherichia coli ATCC 25922, Salmonella typhi ATCC 6539, Shigella dysenteriae ATCC 13313, and non-pathogenic Escherichia coli BAA-1427 applying ciprofloxacin antibiotic as a standard reference, antibacterial study against anaerobic bacteria which include Bacteroides fragilis ATCC 25285, Clostridium perfringens ATCC 13124, Fusobacterium necrophorum ATCC 25286, and Prevotella melaninogenica ATCC 25845 employing metronidazole as a reference, and antifungal study versus Candida albicans ATCC 10231 and Aspergillus niger ATCC 16888, nystatin was the reference. The toxicity against the normal bacterial flora was evaluated to verify the safety profile of the extracts. The results revealed that the extracts have antimicrobial activities with supremacy linked to ethanol extract. In accordance to the activity evidence values, the extracts displayed bactericidal and fungicidal activities, with ethanol extract having the least harmful effect against non-pathogenic microbial strain.