Document Type: Short communication


1 Department of Chemistry, Faculty of Basic Sciences, GonbadKavous University, GonbadKavous, Iran

2 Research Center for Conservation of Culture Relics (RCCCR), Research institute of Cultural Heritage & Tourism, Tehran, Iran

3 Department of Rangeland Science, GonbadKavous University, GonbadKavous, Iran

4 Department. Plant Sciences - Weed Eco physiology, GonbadKavous University, GonbadKavous, Iran


Anbarnesa or donkey dung is one of the most effective smokes used for treatment of many infectious and viral diseases. It is also used as disinfectants. The purpose of this study was to evaluate the antimicrobial and anti-radical activity of Anbarnesa smoke and to provide ointment. In this research study, the donkey dung was burned by the designed machine and the smoke was collected in a diethyl ether solvent. The solvent evaporated at environment temperature and was used to test the dried specimens. To determine the MIC and MBC of the sample at the presence of bacteria was used Macro broth dilution method. The MIC and MBC of the smoke was shown on Staphylococcus aureus 1.87, 3.75 mg/mL and on Escherichia coli 3.75, 7.5 mg/mL, respectively. The average percentage of the inhibitation of free radicals in concentration of 9, 6, and 3% of smoke, was found to be 28.37, 18.86 and 8.86, respectively.

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