@article { author = {Yadav, C. Hazarathaiah and Mallibabu, A.}, title = {RP-LC Method in Gradient mode for Combined Quantification of Metformin, Linagliptin, and Empagliflozin in Combination Tablets}, journal = {Journal of Medicinal and Chemical Sciences}, volume = {5}, number = {6}, pages = {1018-1025}, year = {2022}, publisher = {Sami Publishing Company (SPC)}, issn = {2651-4702}, eissn = {2651-4702}, doi = {10.26655/JMCHEMSCI.2022.6.15}, abstract = {It has been developed and validated an accurate, sensitive, precise, quick, and gradient reverse phase HPLC (RP-HPLC) technique for combined quantification of Metformin, Linagliptin, and Empagliflozin in raw materials and pharmaceutical combined tablets. With acetonitrile as the organic solvent, the best separation was achieved on a 250 mm x 4.6 mm i.d., 5µ-particle size Inertsil®-Octadecyl-silyl-3V-Reverse-Phase-C18-column with acetonitrile as the non-polar-modifier and Dipotassium mono-hydrogen phosphate buffers system [0.03M] in water with pH 3.0 adjusted with ortho-phosphoric acid (0.1 %v/v) in the gradient mode of elution as an elution solvent at a speed of 1.0 mL.min−1. UV detection was at 230-nm. Metformin had a retention time of 2.421 minutes, Linagliptin's was 8.187 minutes, and Empagliflozin's was 11.71 minutes. With a correlation coefficient of about 0.9999, the peak-response was obtained as a function of concentration over the range of 80-480 mcg/mL for metformin, 0.4-2.4 mcg/mL for linagliptin, and 0.8-4.8 mcg/mL for empagliflozin. Metformin, Linagliptin, and Empagliflozin were shown to have a percentage assay of 99.92, 99.72, and 99.74, respectively. Metformin, Linagliptin, and Empagliflozin, each have a limit of detection of 0.04 g/mL, 0.004 g/mL, and 0.004 g/mL, respectively. Metformin, Linagliptin, and Empagliflozin, each have a limit of quantification (LOQ) of 0.12 ng/mL, 0.012 g/mL, and 0.012 g/mL, respectively. The presence of excipients in the formulation had no effect on the assay method. The procedure is appropriate for usage in QC- laboratories since it is quick and precise.}, keywords = {Metformin,Linagliptin,Empagliflozin,Gradient-RP-HPLC,and Fixed dosage forms}, url = {https://www.jmchemsci.com/article_149694.html}, eprint = {https://www.jmchemsci.com/article_149694_1518989c5d5739ea9634507ee29b934f.pdf} }