Journal of Medicinal and Chemical Sciences

Background: Pseudomonas aeruginosa is the most common cause of bacterial keratitis, with potential complications such as corneal perforation and endophthalmitis. Platelet-rich fibrin ... Read More Background: Pseudomonas aeruginosa is the most common cause of bacterial keratitis, with potential complications such as corneal perforation and endophthalmitis. Platelet-rich fibrin (PRF) membrane is utilized as a treatment for corneal ulcers. Maintaining a balance between collagen formation and extracellular matrix remodeling is crucial in fibrosis, a process regulated by matrix metalloproteinases (MMPs) and Tissue Inhibitors of Matrix Metalloproteinases (TIMPs). This study aims to analyze the effect of the PRF membrane on the expression of MMP-9 and TIMP-1 in keratitis.Methods: This study employed an experimental design with a randomized posttest-only control group. New Zealand white rabbits (Oryctolagus cuniculus) were used as animal models with Pseudomonas aeruginosa keratitis. Levofloxacin eye drops were administered to both the control and treatment groups. However, in the treatment group, a PRF membrane was sutured. The expression of MMP-9 and TIMP-1 was compared between the two groups.Results: The animal models consisted of 12 New Zealand white rabbits, divided into a control group and a treatment group, each comprising 6 rabbits. The mean ± SD of MMP-9 in the treatment group was 2.58 ± 0.72, whereas in the control group, it was 4.62 ± 1.56. TIMP-1 data in the treatment group had a median of 4.10 (with a minimum of 2.00 and a maximum of 4.80), while in the control group, it was 4.80 (with a minimum of 4.20 and a maximum of 8.96). Analysis using the independent t-test and Mann-Whitney test revealed substantial disparities in the expression levels of MMP-9 and TIMP-1 between the control and treatment groups, with p-values of 0.016 and 0.036, respectively. The treatment group exhibited a lower expression of MMP-9 compared to the control group, whereas the treatment group demonstrated a higher expression of TIMP-1 in comparison to the control group.Conclusion: PRF membranes can modulate MMP-9 expression and the balance between MMP-9 and TIMP-1 in degrading and depositing the extracellular matrix (ECM). They can be considered as an option for cases of Pseudomonas aeruginosa keratitis to reduce corneal scarring.

Relationship between Adjuvant Thiamine, Ascorbic Acid, and Combinations of These Drugs with Reducing the Incidence, Increasing MMP-9/TIMP-1 Ratio, and Decreasing Score Sofa in Mechanically Ventilated Patients with Severe Sepsis or Septic Shock

Agus Prima; Rr Sinta Irina; Bastian Lubis

Volume 6, Issue 5 , May 2023, , Pages 1055-1064

https://doi.org/10.26655/JMCHEMSCI.2023.5.11

Abstract

A high number of sepsis patients demand all health institutions to provide adequate treatment and management. Several therapeutic agents are developed as adjuvants for handling sepsis ... Read More

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Keywords = 9 TIMP

Effect of Platelet-Rich Fibrin (PRF) Membrane on the Expression of Matrix Metalloproteinase 9 (MMP-9) and Tissue Inhibitor of Metalloproteinase 1 (TIMP-1) in Corneal Wound Healing: Pseudomonas aeruginosa Keratitis

Abstract

Relationship between Adjuvant Thiamine, Ascorbic Acid, and Combinations of These Drugs with Reducing the Incidence, Increasing MMP-9/TIMP-1 Ratio, and Decreasing Score Sofa in Mechanically Ventilated Patients with Severe Sepsis or Septic Shock

Abstract